Fig. 3: MiR-15b and miR-322 repress SETD3 expression in vivo.
From: MiR-15b and miR-322 inhibit SETD3 expression to repress muscle cell differentiation
a Transcriptional and protein levels of SETD3 in C2C12 cells expressing various miRNA mimics were examined by RT-qPCR (left panel) and western blot (right panel) analyses. b Sequences of the wild-type or mutants of miR-15b and miR-322 were shown. “*“ indicates the mutated sites. c, d SETD3 protein levels in cells expressing the indicated miRNA mimics or mutants shown in b were examined by western blot (left panel). The relative SETD3 levels normalized to β-Actin were quantified using ImageJ (right panel). e SETD3 protein levels in cells stably expressing the indicated pri-miRNAs were examined by western blot. f, g Transcriptional levels and protein levels of SETD3 in cells transfected the indicated miRNA inhibitors were examined by RT-qPCR (f) and western blot analysis (g, left panel). The relative SETD3 protein levels were quantified (g, right panel). h, i Transcriptional levels of miRNAs in cells transfected with sgRNA targeting miR-15b or miR-322 were examined by RT-qPCR in h, and protein levels of SETD3 were examined by western blot in i. The error bars in each quantification plot represent mean ± SD from three biological repeats
