Fig. 5: MiR-15b and miR-322 inhibit SETD3 to regulate muscle cell differentiation.

a, b C2C12 cells transfected with the indicated sgRNAs (a) or miRNA inhibitors (b) targeting miR-15b or miR-322 were differentiated for 4 days, and the levels of the indicated proteins were examined by western blot. c Immunofluorescence analyses of the indicated differentiated C2C12 cells were stained with antibodies against SETD3 (green) and MHC (red). DAPI stains nuclei. Scale bar: 100 μm. d MHC-positive cell numbers in certain field of the indicated C2C12 cells describe in c were quantified. e Quantitative analyses of nuclei number per fiber from the indicated C2C12 cells. Data represent the mean ± SD from five fields. f Knockdown of SETD3 rescues miR-15b or miR-322 mediated myoblast differentiation. The protein levels of MHC and SETD3 were examined by western blot. S.E. short exposure, L.E. long exposure