Fig. 6: E2F1 and FAM3B regulate SETD3 levels through controlling miR-15b or miR-322 expression.
From: MiR-15b and miR-322 inhibit SETD3 expression to repress muscle cell differentiation
a The transcriptional levels of the indicated genes during muscle differentiation were examined by RT-qPCR. b, c The transcriptional levels of miR-15b or miR-322 after knockdown E2F1 (b) or knockdown FAM3B (c) were examined. The relative SETD3 levels were shown. d, e Western blot assays were performed to examine SETD3 levels after overexpression of E2F1 (d, left panel) or FAM3B (e, left panel) in C2C12 cells. Data represent the mean ± SD from three independent experiments (right panel). f Knockdown of FAM3B by two different siRNAs reduces SETD3 levels. g, i C2C12 cells were transfected with siRNAs targeting E2F1 or FAM3B, respectively, and induced to differentiate for 3 days. The indicated miRNAs or mRNA levels were examined by RT-qPCR. Data represent the mean ± SD from three independent experiments. h, j The same set of cells described in panel g, i were subjected to immunoblotting against the indicated antibodies. S.E. short exposure, L.E. long exposure
