Fig. 2: Lobaplatin induced mature IL-1β release independent of both signal 1 and 2 of the NLRP3 inflammasome. | Cell Death & Disease

Fig. 2: Lobaplatin induced mature IL-1β release independent of both signal 1 and 2 of the NLRP3 inflammasome.

From: Cleavage of GSDME by caspase-3 determines lobaplatin-induced pyroptosis in colon cancer cells

Fig. 2

The mRNA levels of IL-1β, CASP-1, ASC and NLRP3 in HT-29 (a) and HCT116 (b) cells treated with lobaplatin (16 μg/ml) for 8 h was detected by qRT-PCR. Supernatants (SN) and cell extracts (Lysate) were analyzed by western blotting analysis (c, d). ELISA for IL-1β and LDH release of HT-29 (e, f) and HCT116 (g, h) cells treated with lobaplatin in the presence or absence of Z-YVAD-FMK or siRNA-NLPR3 transfection. All the data are presented as the mean ± SD from three independent experiments. *p < 0.05 vs. control using one-way ANOVA

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