Fig. 3: GSDME, rather than GSDMD, is cleaved during lobaplatin-induced pyroptosis in colon cancer cells. | Cell Death & Disease

Fig. 3: GSDME, rather than GSDMD, is cleaved during lobaplatin-induced pyroptosis in colon cancer cells.

From: Cleavage of GSDME by caspase-3 determines lobaplatin-induced pyroptosis in colon cancer cells

Fig. 3

Gel images of GSDMD-C terminus (GSDMD-C) in HT-29 and HCT116 cells treated with different doses (0, 8, 16 and 32 μg/ml) of lobaplatin (a, b). ELISA for IL-1β (c) and LDH (d) release of HT-29 and HCT116 cells treated with lobaplatin in the presence or absence of GSDMD knockdown. Gel images of GSDME-N in HT-29 (e) and HCT116 (f) cells treated with different doses (0, 8, 16 and 32 μg/ml) of lobaplatin. Gel images of pro-caspase-3/6/7 and cleaved-caspase-3/6/7 in HT-29 (g) and HCT116 (h) cells treated with of lobaplatin (16 μg/ml). All the data are presented as the mean ± SD from three independent experiments. *p < 0.05 vs. control using one-way ANOVA

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