Table 1 Primer sequence
From: Cleavage of GSDME by caspase-3 determines lobaplatin-induced pyroptosis in colon cancer cells
Gene | sequence |
|---|---|
IL-1β for qRT-PCR | F: 5′- AGCTACGAATCTCCGACCAC -3′ |
R: 5′- CGTTATCCCATGTGTCGAAGAA -3′ | |
CASP-1 for qRT-PCR | F: 5′- TTTCCGCAAGGTTCGATTTTCA -3′ |
R:5′- GGCATCTGCGCTCTACCATC -3′ | |
NLRP3 for qRT-PCR | F: 5′- CGTGAGTCCCATTAAGATGGAGT -3′ |
R: 5′- CCCGACAGTGGATATAGAACAGA -3′ | |
ASC for qRT-PCR | F: 5′- TGGATGCTCTGTACGGGAAG -3′ |
R: 5′- CCAGGCTGGTGTGAAACTGAA -3′ | |
GAPDH for qRT-PCR | F: 5′-TGCACCACCAACTGCTTAGC -3′ |
R: 5′-GGCATGGACTGTGGTCATGAG -3′ |
