Fig. 3: MiR-184 upregulates the expression of Fas in HTR8 cells.

a We detected some molecules related to the poptotic pathway by western blot analysis, including caspase-9, -8, -3, -7, Fas, and FasL, and only the expression of Fas was changed after miR-184 overexpression. b Flow cytometry was used to examine the expression of Fas on NC and miR-184 cells, showing that the percentage of Fas-positive cells was increased in miR-184 cells, as well as the mean fluorescence intensity. Unpaired student’s t-test (two-tailed) was used for comparison between two groups. ****P < 0.0001. Data represent mean ± SEM. c Meanwhile, RT-PCR was applied to detect the mRNA level of Fas in two groups. Unpaired student’s t-test (two-tailed) was used for comparison between two groups. **P < 0.01. Data represent mean ± SEM. d After stimulation with various concentrations of anti-Fas activating antibody (zero, 1, 3, 5 μg /mL) for 24 h, the apoptosis rate of NC and miR-184 cells was detected. Two-way ANOVA was used for comparison between these groups. **P < 0.01, ***P < 0.001, ****P < 0.0001. Data represent mean ± SEM. e The protein level of caspase-3, -8, and cleaved caspase-3, -8 in NC and miR-184 cells after stimulation with different concentrations of anti-Fas activating antibody (zero, 3, 5 μg /mL) was also examined by western blot analysis. These experiments were repeated three times. (miR-184: HTR8 cells infected with miR-184 overexpression lentivirus; NC: HTR8 cells infected with negative control lentivirus.)