Fig. 5: Luteolin suppressed AIM2 inflammasome through downregulating the expression of AIM2 in A549 and H460 cells.
From: Decrease of AIM2 mediated by luteolin contributes to non-small cell lung cancer treatment
a Western blot analysis of AIM2 expression in A549 and H460 cells transfected with siAIM2 or AIM2 overexpression plasmid. Cells were transfected with siAIM2 or AIM2 overexpression plasmid, with or without the treatment of luteolin (40 μM) for 24 h. b The expressions of AIM2, pro-caspase-1, caspase-1 p10, pro-IL-1β and IL-1β were determined by western blot. c RT-qPCR were performed to detect the mRNA levels of AIM2, caspase-1 and IL-1β. d Cells were pretreated with siAIM2 or AIM2 overexpression plasmid before treating with or without luteolin for 24 h to detect the localization of ASC by immunofluorescence (scale bar = 25 μm). *P < 0.05, **P < 0.01, ***P < 0.001 vs control; #P < 0.05, ##P < 0.01, ###P < 0.001 vs luteolin (40 μM). NS no significant difference
