Fig. 5: miR-4284 is decreased in ASMSCs and regulates expression of CXCL5. | Cell Death & Disease

Fig. 5: miR-4284 is decreased in ASMSCs and regulates expression of CXCL5.

From: Abnormal inhibition of osteoclastogenesis by mesenchymal stem cells through the miR-4284/CXCL5 axis in ankylosing spondylitis

Fig. 5

a Bioinformatics analysis in three databases (TargetScan, miRBase, and miRDB) to identify possible miRNAs targeting CXCL5. Seven miRNAs with the highest scores for targeting CXCL5 are shown. b Expression levels of miRNAs in HDMSCs and ASMSCs were determined by qRT-PCR. c Expression levels of miR-4284 in HDMSCs and ASMSCs after treatment with miR-4284 inhibitor or mimic. d Expression levels of CXCL5 in HDMSCs and ASMSCs after treatment with miR-4284 inhibitor or mimic. e Possible binding sites between miR-4284 and CXCL5 are shown, and a mutant (MUT) CXCL5 site was constructed. f Binding sites were confirmed by luciferase assays. The miR-4284 mimic decreased the luciferase activity of wild-type (WT) CXCL5 but had no impact on MUT CXCL5. Data are presented as the mean ± SD (n = 30). The results represent three independent experiments. *, p < 0.05; HDMSCs, mesenchymal stem cells from healthy donors; ASMSCs, mesenchymal stem cells from patients with ankylosing spondylitis

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