Fig. 6: miR-4284 can regulate MSC-mediated inhibition of osteoclastogenesis.

a Expression of miR-4284 was determined by qRT-PCR on day 3 after treatment with the miR-4284 inhibitor. b Representative images of TRAP staining of osteoclasts co-cultured with MSCs on day 9 after treatment with the miR-4284 inhibitor (× 100). c The number of TRAP⁺ osteoclasts in each well is shown. d Representative images of osteoclasts stained with FITC-phalloidin on day 9 (× 200). e Representative images of bone resorption assays on day 15 (× 200). f Pit formation on each slide was assessed. g Expression of miR-4284 was determined by qRT-PCR on day 3 after treatment with the miR-4284 mimic. h Representative images of TRAP staining of osteoclasts co-cultured with MSCs after treatment with the miR-4284 mimic on day 9 (× 100). i The number of TRAP⁺ osteoclasts in each well is shown. j Representative images of osteoclasts stained with FITC-phalloidin on day 9 (× 200). k Representative images of bone resorption assays on day 15 (× 200). l Pit formation on each slide was assessed. Data are presented as the mean ± SD (n = 30). The results represent three independent experiments. *, p < 0.05; HDMSCs, mesenchymal stem cells from healthy donors; ASMSCs, mesenchymal stem cells from patients with ankylosing spondylitis