Fig. 7: Macrophage phenotypic alterations induced by WT and Gpr174-deficient mouse Treg cells.

a A representative flow cytometry result of F4/80⁺CD11b⁺ macrophage stained with anti-CD206 and MHC-II. Phenotype of macrophage characteristics of septic mice that received none, PC61 injection (200 μg/mouse, 3 days before LPS injection) and Treg cells adaptive transfer (16 h before LPS injection). b Quantification of CD206 and MHC-II in macrophages of physiological status mice. c Quantification of CD206 and MHC-II in macrophages of LPS challenged mice (24 h). d Quantification of CD206 and MHC-II in macrophages of LPS challenged (24 h) mice that received PC61 injection. e Quantification of CD206 and MHC-II in macrophages of LPS challenged (24 h) mice after Treg cells adoptive transfer. Data are representative of three independent experiments (n = 3 mice/group). Data are  shown as mean ± S.D. *P < 0.05; **P < 0.01