Fig. 4: NPTX2 activates Wnt/β-catenin signaling and promotes the nuclear translocation of β-catenin. | Cell Death & Disease

Fig. 4: NPTX2 activates Wnt/β-catenin signaling and promotes the nuclear translocation of β-catenin.

From: NPTX2 promotes colorectal cancer growth and liver metastasis by the activation of the canonical Wnt/β-catenin pathway via FZD6

Fig. 4

a GSEA analysis of NPTX2 expression in CRC, as evaluated from TCGA dataset. A total of 286 CRC samples were divided into two groups, including high NPTX2 expression group (143 samples) and low NPTX2 expression group (143 samples). b The fluorescence intensity of Wnt-β-catenin signaling after NPTX2 knockdown or overexpression was evaluated with a luciferase reporter assay. c Total β-catenin expression in SW480 and LoVo cells transfected with sh-NPTX2, sh-control, vector, and NPTX2-HA (Student’s t test). d Nuclear accumulation of β-catenin after NPTX2 knockdown and overexpression in SW480 and LoVo cells; e β-catenin localization in SW480 and LoVo cells transfected with sh-NPTX2 or sh-control. f β-catenin expression and localization in an orthotopic model of CRC injected with SW480 cells (sh-NPTX2 [n = 6], sh-control [n = 6], vector [n = 6], and NPTX2-HA [n = 6]). g β-catenin expression and localization in a liver metastasis model of CRC injected with SW480 cells (sh-NPTX2 [n = 6], sh-control [n = 6], vector [n = 6], and NPTX2-HA [n = 6]). *P < 0.05. All experiments were performed in triplicates. Measurement data were presented as the mean ± SD. *P < 0.05

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