Fig. 6: FZD6 is indispensable for the NPTX2-mediated activation of Wnt/β-catenin signaling in CRC.

a Viability of NPTX2-HA SW480 and LoVo cells transfected with si-control and si-FZD6 (Student’s t test). b Colony-formation ability of NPTX2-HA SW480 and LoVo cells transfected with si-control and si-FZD6. c Cell migration ability of NPTX2-HA SW480 and LoVo cells transfected with si-control and si-FZD6. d Colony-formation ability of NPTX2-HA SW480 and LoVo cells transfected with si-control and si-FZD6 (Student’s t test). e Number of migratory cells among NPTX2-HA SW480 and LoVo cells transfected with si-control and si-FZD6 (Student’s t test). f, g β-catenin level in the nucleus of NPTX2-HA SW480 and LoVo cells transfected with si-control and si-FZD6. H and I. Protein expression of β-catenin target genes in NPTX2-HA SW480 and LoVo cells transfected with si-control and si-FZD6. In all the graphs relative to experiments with cell lines, three replicates were evaluated. Measurement data were presented as the mean ± SD. *P < 0.05