Fig. 1: Identification of new necroptosis inhibitors.

a Schematic overview of the drug screen workflow. b Chemical structure of GSK’067 and GSK’074. c Mouse smooth muscle cell line MOVAS were treated with 30 ng/ml TNFα plus 60 μM zVAD and indicated compounds for 6 h. Cell viability was detected by CellTiter-Glo. Data were normalized to DMSO treated control cells and presented as mean ± S.D. n = 3. d Mouse fibroblast cell line L929 were treated with 20 ng/ml TNFα plus 40 μM zVAD and GSK’067, GSK’074, or GSK’843 for 3 h. Cell viability was detected by CellTiter-Glo. Data were normalized to DMSO treated control cells and presented as mean ± S.D. of three independent experiments