Fig. 7: GSK’074 attenuates cell death and inflammation in calcium phosphate injured aortae. | Cell Death & Disease

Fig. 7: GSK’074 attenuates cell death and inflammation in calcium phosphate injured aortae.

From: Identification of a novel class of RIP1/RIP3 dual inhibitors that impede cell death and inflammation in mouse abdominal aortic aneurysm models

Fig. 7

a Experimental design of the calcium phosphate aneurysm model. Mice were treated with 200 µl vehicle (8% DMSO) or GSK’074 at 0.93 mg/kg/day immediately following aneurysm induction. Three male mice were in each group. Mice were euthanized 4 days after. b, e Representative photographs of immunostaining for markers smooth muscle cell (SM-αActin) or macrophages (CD68). Scale bar = 50 μm. c Mice were injected with propidium iodide (PI) 2 h before euthanization. DAPI was used to stain nuclei. Quantification of PI-positive cells in the medial layer (indicated by white dashed line) was shown on the right. Scale bar = 50 μm. d Cross-sections of aneurysm prone tissue were immunostained with anti-MLKL serine345 phosphorylation (p-MLKL S345) as a marker of necroptosis. Scale bar = 50 μm. f Representative photographs of terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) in aortic cross-sections. Quantification of TUNEL-positive cells percentage was shown on the right. Scale bar = 50 μm. *P < 0.05

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