Fig. 1: Long noncoding RNA (LncRNA) SMAD5-AS1 is down-regulated in diffuse large B cell lymphoma (DLBCL) tissues and cell lines. | Cell Death & Disease

Fig. 1: Long noncoding RNA (LncRNA) SMAD5-AS1 is down-regulated in diffuse large B cell lymphoma (DLBCL) tissues and cell lines.

From: Lnc SMAD5-AS1 as ceRNA inhibit proliferation of diffuse large B cell lymphoma via Wnt/β-catenin pathway by sponging miR-135b-5p to elevate expression of APC

Fig. 1

a The heat map with hierarchical cluster of the top 40 differentially expressed lncRNAs between DLBCL samples and normal samples (>1.5-fold; P < 0.05). b LncRNAs with fold change >1.5 and P < 0.05 were plotted in the volcano plot. A volcano plot is constructed by plotting the negative log of the P value on the y-axis. These result in data points with low P values appearing toward the top of the plot. The x-axis is the log of the fold change between the DLBCL and normal samples. c In situ hybridization detection of SMAD5-AS1 in DLBCL tissue and adjacent normal tissue (×100 and ×400). d Real-time quantitative polymerase chain reaction (RT-qPCR) was performed to validate SMAD5-AS1 expression in DLBCL samples and normal samples (n = 11 vs. 11). ***P < 0.001. The expression of SMAD5-AS1 was lower in tumor tissues than that in normal tissues. e RT-qPCR was performed to measure the relative expression of SMAD5-AS1 in several lymphoma cell lines and normal human B lymph cells (GM12878). The majority of SMAD5-AS1 was located in the cytoplasm according to the nuclear mass separation assay (f) and fluorescence in situ hybridization (g)

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