Fig. 2: SMAD5-AS1 inhibits cell proliferation of diffuse large B cell lymphoma (DLBCL) cells in vitro. | Cell Death & Disease

Fig. 2: SMAD5-AS1 inhibits cell proliferation of diffuse large B cell lymphoma (DLBCL) cells in vitro.

From: Lnc SMAD5-AS1 as ceRNA inhibit proliferation of diffuse large B cell lymphoma via Wnt/β-catenin pathway by sponging miR-135b-5p to elevate expression of APC

Fig. 2

a Efficiency of SMAD5-AS1 expression in SMAD5-AS1-overexpressed TMD8 and U2932 cells was evaluated by real-time quantitative polymerase chain reaction (RT-qPCR). The expression of SMAD5-AS1 in SMAD5-AS1-overexpressed group was obviously increased compared with the control group. ***P < 0.001. Cell proliferation and viability were measured by Cell Counting Kit-8 (CCK8) (b) and MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) (c) assays. The cell proliferation and viability were reduced in SMAD5-AS1-overexpressed group compared with that in the control group. *P < 0.01. d The phase of the cell cycle was detected by fluorescence-activated cell sorting (FACS) analysis. Pictures of flow cytometry were shown in the upper panel. (The area under the left red peak chart represents the proportion of cells in the G1 phase. The area under the right red peak chart represents the proportion of cells in the G2/M phase. The area of oblique line represents the proportion of cells in the S phase.), while comparison was performed in the corresponding histograms of lower panels. The number of cells in the G1/G0 phase was increased in the SMAD5-AS1-overexpressed group, while that in the G2/M phase was declined. **P < 0.01. e Annexin V assay was used to determine cell apoptosis rate. Overexpression of SMAD5-AS1 promoted apoptosis of lymphoma cells. **P < 0.01. N = 3 independent experiments

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