Fig. 3: Down-regulated SMAD5-AS1 promotes cell proliferation of diffuse large B cell lymphoma (DLBCL) cells in vitro. | Cell Death & Disease

Fig. 3: Down-regulated SMAD5-AS1 promotes cell proliferation of diffuse large B cell lymphoma (DLBCL) cells in vitro.

From: Lnc SMAD5-AS1 as ceRNA inhibit proliferation of diffuse large B cell lymphoma via Wnt/β-catenin pathway by sponging miR-135b-5p to elevate expression of APC

Fig. 3

a Efficiency of SMAD5-AS1 expression in SMAD5-AS1 down-regulated TMD8 and U2932 cells was evaluated by real-time quantitative polymerase chain reaction (RT-qPCR). The expression of SMAD5-AS1 in shSMAD5-AS1 group was obviously decreased compared with control group. ***P < 0.001. Cell proliferation and viability were measured by Cell Counting Kit-8 (CCK8) (b) and MTT (c) assays. The cell proliferation and viability were enhanced in shSMAD5-AS1 group compared with that in control group. *P < 0.01. d The phase of the cell cycle was detected by FACS analysis. Pictures of flow cytometry were showed in the upper panel, while comparison was performed in the corresponding histograms of lower panels. The number of cells in the G1/G0 phase was decreased in shSMAD5-AS1 group, while that in the G2/M phase was elevated **P < 0.01. e Annexin V assay was used to determine cell apoptosis rate. Down-regulation of SMAD5-AS1 inhibited apoptosis of lymphoma cells **P < 0.01. N = 3 independent experiments

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