Fig. 6: Adenomatous polyposis coli (APC) is identified as a direct target of miR-135b-5p in diffuse large B cell lymphoma (DLBCL) proliferation. | Cell Death & Disease

Fig. 6: Adenomatous polyposis coli (APC) is identified as a direct target of miR-135b-5p in diffuse large B cell lymphoma (DLBCL) proliferation.

From: Lnc SMAD5-AS1 as ceRNA inhibit proliferation of diffuse large B cell lymphoma via Wnt/β-catenin pathway by sponging miR-135b-5p to elevate expression of APC

Fig. 6

a Schematic diagram showed the predicted miR-135b-5p binding sites with the 3′-untranslated region (UTR) of APC. The sequences of wild-type and mutant 3′-UTR of APC were listed as well. Luciferase reporter gene assays were performed to measure the luciferase activity in TMD8 cells. **P < 0.01. b The relative expression of APC in DLBCL cells transfected with SMAD5-AS1-overexpressed plasmid/control plasmid and shSMAD5-AS1 plasmid/control plasmid. c Real-time quantitative polymerase chain reaction (RT-qPCR) and Cell Counting Kit-8 (CCK8) assay were applied to measure the relative expression of APC and cell proliferation in TMD8 cells at 36 h after transfection with control, miR-135b mimic or miR-135b inhibitor, APC overexpression plasmid or shAPC plasmid, and miR-135b mimic + Lv-APC or miR-135b inhibitor + shAPC. *P < 0.05. N = 3 independent experiments

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