Fig. 1: SFN-Cys inhibited migration and invasion via sustained ERK1/2 phosphorylation. | Cell Death & Disease

Fig. 1: SFN-Cys inhibited migration and invasion via sustained ERK1/2 phosphorylation.

From: Sulforaphane metabolites inhibit migration and invasion via microtubule-mediated Claudins dysfunction or inhibition of autolysosome formation in human non-small cell lung cancer cells

Fig. 1

a Cells were treated with increasing concentrations of SFN-Cys for 24 h, then cell viability was determined by Cell Proliferation Assay Kit and presented as the percentages vs. the control. b Cells were scratched and treated with SFN-Cys at the indicated concentrations for 24 h. An image of the wound closure area was captured at × 40 magnification and measured with ImageJ software. c The cells (1 × 105) were seeded in 24-well invasion chambers and treated with increasing doses of SFN-Cys. Cells were stained with crystal violet, images were taken at ×100 magnification and analyzed. d Western blotting was used to analyze pERK1/2 expression. e Cells were pretreated with PD98059 (25 µM) for 30 min, then treated with SFN-Cys (10 µM) for 24 h. The expression of phosphorylated ERK1/2 was analyzed by Western blotting. f Cells were treated with phosphorylated ERK1/2 inhibitor PD98059 (25 µM) for 30 min, then 10 µM of SFN-Cys was added to the medium for 24 h. The invaded cells were counted. *P < 0.05, **P < 0.01. Data were shown as means ± SD (n = 3)

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