Fig. 2: SFN-Cys regulated Claudin-1, 5, and 7 in A549 and SK-1 cells via phosphorylated ERK1/2.

a Survival analysis in Claudin-5-expressed patients via GEPIA database and the interactions among Claudins and related partners were analyzed via the String net. (CLDN1: Claudin-1; CLDN5: Claudin-5; CLDN7: Claudin-7; TJP1: ZO-1; OCLN: Occludin; F11R: Junction adhesion molecule A) were shown. b Expressions of Claudin-1, 5, and 7 were evaluated in the microarray tissues with pathological grades of lung adenocarcinoma and lung squamous carcinoma. A549 cells belong to lung adenocarcinoma and SK-1 cells belong to lung squamous carcinoma. IHC of Claudin-1, 5, and 7 in adjacent and cancer tissues with three malignant grades were used (AT: adjacent tissues; TT: tumors tissues). Magnification × 200, Scale bars: 100 µm. c Western blotting was used to analyze Claudin-1, 5, and 7 expression in the cells treated with SFN-Cys at the indicated concentrations. d Cells were pretreated with PD98059 (25 µM) for 30 min, then treated with SFN-Cys (10 µM) for 24 h. The expressions of Claudin-1, 5, and 7 were analyzed by Western blotting. *P < 0.05; **P < 0.01; ***P < 0.001. Data were shown as means ± SD (n = 3)