Fig. 3: Knockdown of Claudin-1, 5, and 7 suppressed significantly migration and invasion in NCSLC cells. | Cell Death & Disease

Fig. 3: Knockdown of Claudin-1, 5, and 7 suppressed significantly migration and invasion in NCSLC cells.

From: Sulforaphane metabolites inhibit migration and invasion via microtubule-mediated Claudins dysfunction or inhibition of autolysosome formation in human non-small cell lung cancer cells

Fig. 3

a Cells were transfected with normal control (NC) siRNA or Claudin-1, 5, and 7 siRNAs (Con: control; T1: transfection Claudin-1; T5: transfection Claudin-5; T7: transfection Claudin-7). After transfection for 48 h, the cells were seeded in six-well plates and treated with SFN-Cys for 24 h. Then the cells lysates were collected and the expressions of Claudin-1, 5, and 7 were analyzed by Western blotting. b Both A549 and SK-1 cells transfected with NC siRNA or Claudin-1, 5, and 7 siRNA were seeded in 24-well invasion chambers to detect invasion. *P < 0.05; **P < 0.01. Data were shown as means ± SD (n = 3)

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