Fig. 4: p27 and p18 are dispensable for genomic stability during reprogramming. | Cell Death & Disease

Fig. 4: p27 and p18 are dispensable for genomic stability during reprogramming.

From: Absence of cyclin-dependent kinase inhibitor p27 or p18 increases efficiency of iPSC generation without induction of iPSC genomic instability

Fig. 4

a Representative images of γ-H2AX staining in various genotypes cells at reprogramming Day 0, Day 4, and Day 12. Bars: 50 μm. b Percentage of cells with γ-H2AX foci in (a). c Representative images of comet assay in various genotype cells at reprogramming Day 12. Bars: 200 μm. d Tail intensity of the cells in (c). e The karyotypic alterations in three clones each from three independent iPSCs at passage 5 derived from three passage 3 MEF cell lines per genotype were quantified. Graphic illustration of the number of chromosomal alterations (numerical gains, losses, and structural alterations) in the iPSCs. f Karyotypes from 20 metaphase cells from three sets of each three cell lines per type of iPSCs were analyzed under the microscope or after digital imaging and karyotyping. Images shown were taken with ×100 oil objective. Data are representative of two or three independent experiments. Error bars, ±SD. **p < 0.01, ***p < 0.001, by two-tailed t test

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