Fig. 2: C-C chemokine receptor type 5 (CCR5)-mediated cellular responses in vitro.
From: Bone marrow-derived mesenchymal stem cells promote colorectal cancer progression via CCR5

a CCR5 mRNA expression in HCT116 transfectant cells quantified by quantitative reverse transcription-PCR. Means; bar, ±SD, n = 3. b CCR5 cell surface expression (top) and intracellular expression (bottom) analyzed by flow cytometry. c Intracellular signaling of HCT116 transfectant cells treated with C-C motif chemokine ligand 5 (CCL5; 100 ng/ml). d Cell proliferation assay using Cell Counting Kit-8 assay. HCT116 transfectant cells treated with CCL5 (100 ng/ml) analyzed at day 0, 3, and 6. Normalize an absorbance at day 0 as 1. Mean; bars, ±SD, n = 3 (Student’s t test; *P = 0.008, **P = 0.03). e Chemotaxis index of HCT116 transfectant cells treated with various CCL5 concentrations (left). Mean; bars, ±SD, n = 6 (Student’s t test; *P < 0.001). Representative images of transwell membrane (right). Scale bar, 200 μm