Fig. 2: CAF-derived TGF-β promotes TUG1 expression in OS. | Cell Death & Disease

Fig. 2: CAF-derived TGF-β promotes TUG1 expression in OS.

From: Long non-coding RNA Taurine upregulated gene 1 promotes osteosarcoma cell metastasis by mediating HIF-1α via miR-143-5p

Fig. 2

a TGF-β expression levels in OS patients and the normal group were detected by ELISA. b TGF-β expression levels in OS and non-tumour tissues were detected by ELISA. c TGF-β expression levels in OS cell lines, OS CAFs, and normal fibroblasts were quantified by qRT-PCR. d, e Expression levels of TGF-β mRNA and protein in CAFs transfected with TGF-β siRNA (siTGF-β) were detected by qRT-PCR and western blot, respectively. f Diagram of the in vitro co-culture system. g, h Effect of CAF TGF-β knockdown and TGF-β on OS cell (143B and U2OS) TUG1 expression was determined using the in vitro co-culture system. ***P < 0.001; ns, not significant

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