Fig. 3: TUG1 acts as miRNA sponge and negatively regulates miR-143-5p expression. | Cell Death & Disease

Fig. 3: TUG1 acts as miRNA sponge and negatively regulates miR-143-5p expression.

From: Long non-coding RNA Taurine upregulated gene 1 promotes osteosarcoma cell metastasis by mediating HIF-1α via miR-143-5p

Fig. 3

a Sequence alignment of miR-143-5p and TUG1. b Expression levels of miR-143-5p in OS and non-tumour tissues were detected by qRT-PCR. c Correlation between TUG1 and miR-143-5p expression was determined in OS tissues. d TUG1 expression levels in OS cells (143B and U2OS) transfected with TUG1 siRNA (si-TUG1) were detected by qRT-PCR. e Expression levels of miR-143-5p in OS cells (143B and U2OS) transfected with TUG1 siRNA (si-TUG1) were detected by qRT-PCR. f Expression levels of TUG1 in OS cells (143B and U2OS) transfected with miR-143-5p inhibitors were detected by qRT-PCR. g, h miR-143-5p reduced the activity of the luciferase reporter with the TUG1-specific sequence but not the mutant sequence. i Amount of TUG1 bound to Ago2 or IgG measured by RT-qPCR after RIP; IgG was used as a negative control and SNRNP70 was used as a positive control. j OS cells (143B and U2OS) were transfected with biotinylated wild-type miR-143-5p (Bio-miR-143-5p), biotinylated mutant miR-143-5p (Bio-miR-143-5p-mut), or biotinylated NC (Bio-NC). Forty-eight hours after transfection, cells were collected for the biotin-based pull-down assay. TUG1 expression levels were analysed by qRT-PCR. **P < 0.01; ***P < 0.001; ns not significant

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