Fig. 3: BBR inhibits proliferation and migration of HaCaT cells.

a CCK8 assays of HaCaT cells treated with or without BBR. b CCK8 assays of HaCaT cells treated with BBR at different concentrations for 72 h. c Colony formation efficiency of HaCaT cells treated with or without BBR. d HaCaT cells were treated with or without BBR for 48 h and cell cycle distribution was analyzed by flow cytometry. e EdU assays of HaCaT cells treated with or without BBR for 24 h. f HaCaT cells were transfected with HA-tagged CDC6 expression plasmid or control plasmid (HA). Forty-eight hours later, cells were treated with or without 40 μM BBR for another 24 h, and EdU assays were performed. g, h HaCaT cells treated with or without 20 μM BBR for 48 h and indicated mRNA levels were measured by qRT-PCR. i HaCaT cells scraped with a pipette tip were treated with BBR for 24 h and the distances that the cells migrated were detected. i HaCaT cells were treated with BBR for 12 h, and transwell assays were performed. *p < 0.05, **p < 0.01, ***p < 0.001, compared to that of DMSO treated cells