Fig. 5: CT55 is a positive regulator of TNF-α-induced NF-κB signaling. | Cell Death & Disease

Fig. 5: CT55 is a positive regulator of TNF-α-induced NF-κB signaling.

From: Cancer testis antigen 55 deficiency attenuates colitis-associated colorectal cancer by inhibiting NF-κB signaling

Fig. 5

a Luciferase experiments were performed to evaluate TNF-α-induced NF-κB activation in HCT116 cells with or without CT55 overexpression (left). Immunoblot analysis of HCT116 cells transfected with plasmids encoding FLAG-tagged CT55 and FLAG control (right). b Western blot analysis of the indicated protein levels in HCT116 cells transfected with FLAG-CT55 for 24 h and treated with TNF-α (20 ng/ml) for the indicated times. β-Actin served as the loading control. c TNF-α-induced NF-κB luciferase activity in HCT116 cells with or without CT55 (left). Immunoblot analysis of the knockout efficiency (right). d Protein expression levels of the indicated proteins from the HCT116 cells with or without CT55 that were treated with TNF-α (20 ng/ml) for the indicated times were analyzed by western blotting. β-Actin served as the loading control. e The mRNA level associated with the TNF-α-induced transcription of the endogenous TNF-α, IκBα, and c-IAP2 genes in HCT116 cells with or without CT55

Back to article page