Fig. 6: CT55 regulates NF-κB activity by interacting with the IKK complex. | Cell Death & Disease

Fig. 6: CT55 regulates NF-κB activity by interacting with the IKK complex.

From: Cancer testis antigen 55 deficiency attenuates colitis-associated colorectal cancer by inhibiting NF-κB signaling

Fig. 6

a, b Immunoprecipitation and immunoblot analysis of HCT116 cells transfected with FLAG-tagged CT55 and HA-tagged IKKα and IKKβ for 24 h and treated with TNF-α (20 ng/ml) for 6 h. The lysates were immunoprecipitated with anti-Flag or anti-HA antibody. Then, western blotting was performed to detect the indicated proteins. c Western blot analysis of overexpressed CT55 after immunoprecipitation of IKKα and IKKβ in HCT116 cells treated with TNF-α (20 ng/ml) for 6 h. d Western blot analysis of IKKα and IKKβ after immunoprecipitation of FLAG-CT55 in HCT116 cells treated with TNF-α (20 ng/ml) for 6 h

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