Fig. 4: Baicalein suppresses fibronectin (FN)-induced epithelial–mesenchymal transition (EMT) in a calpain-2-dependent manner. | Cell Death & Disease

Fig. 4: Baicalein suppresses fibronectin (FN)-induced epithelial–mesenchymal transition (EMT) in a calpain-2-dependent manner.

From: Baicalein inhibits fibronectin-induced epithelial–mesenchymal transition by decreasing activation and upregulation of calpain-2

Fig. 4

Overexpression of calpain-2 reversed the inhibitory effects of baicalein on FN-induced EMT response in MCF-10A cells. a Cell migration ability was measured using a wound healing assay (magnification, ×100, scale bars: 100 μm). b Cell invasion was assessed using the Matrigel-coated transwell model (magnification, ×200, scale bars: 75 μm). c EMT markers were detected by western blotting and normalized to GAPDH expression. The results are shown as shown as fold-change compared with control cells. Data are shown as mean ± SEM from three separate experiments. *P < 0.05, **P < 0.01, ***P < 0.001

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