Fig. 2: Knockdown of FSCN1 inhibited cell viability and trans-migration in CAL-27 and SCC-25 cells. | Cell Death & Disease

Fig. 2: Knockdown of FSCN1 inhibited cell viability and trans-migration in CAL-27 and SCC-25 cells.

From: FSCN1 is an effective marker of poor prognosis and a potential therapeutic target in human tongue squamous cell carcinoma

Fig. 2

a The cell viability of the indicated cells knocked down FSCN1 was determined by MTT assay. b Images (upper panel) and quantification (lower panel) of the indicated cells treated with melatonin (14 days) were analyzed in a clonogenic assay. c Images (left panel) and quantification (right panel) of the indicated cells treated with melatonin for 24 h were analyzed in a transwell matrix penetration assay (Scale bars, 100 μm). Scramble (sc): the lentiviral vector with a scrambled sequence that does not target any mRNA. shFSCN #1 and #2: FSCN1 with two unique shRNAs (#1, #2) in CAL-27 or SCC-25 cells. All statistical analyses were performed using Student paired t-test. All statistical tests were two-sided. Data is presented as mean ± S.D. **p < 0.01 vs. the corresponding control groups

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