Fig. 4: The expression of FSCN1 in human tongue squamous cell carcinoma (TSCC).

a Expression of FSCN1 mRNA in paired TSCC tissues (N = 48) was measured by qRT-PCR assay with primers with overlapping introns (Primer 2) or non-overlapping introns (Primer 1). The relative FSCN1 mRNA expression levels significantly increased in TSCC tissues (T) compared with those of the matched adjacent normal tissues (N) (p < 0.0001). b Expression of Fascin in paired TSCC tissueswas measured by western blotting (N = 8). The relative FSCN1 protein expression levels significantly increased in TSCC tissues (T) compared with those of the matched adjacent non-carcinoma tissues (ANT) (p < 0.0001). β-Actin was measured as the loading control. c Representative immunohistochemical staining showing positive staining in TSCC tissues with different stages and negative staining in normal tongue tissue(NT) (Scale bars, 100 μm). All statistical analyses were performed using Student paired t-test. All statistical tests were two-sided