Fig. 1: Experimental plan and migration of transplanted hNSCs. | Cell Death & Disease

Fig. 1: Experimental plan and migration of transplanted hNSCs.

From: Transplantation of clinical-grade human neural stem cells reduces neuroinflammation, prolongs survival and delays disease progression in the SOD1 rats

Fig. 1

a Experimental plan: rats were transplanted with hNSCs or HBSS when showed the initial symptoms of the disease (ESS), age-matched not-transplated controls were also analysed. Behavioural test was performed for all the durations of animals life. Histological analysis of the spinal cord was performed at 15DAT, 40DAT and at E-ST, that approximately correspond to MSS, LSS and E-ST. b Schematic showing the transplant strategy and migration of hNSCs (green dots). c Schematic representation of the spinal cord organisation. hNSC or HBSS were transplanted into the anterior horns (asterisk) and were able to radially migrate into the anterior funiculus (red) and the lateral funiculus (blue). d Injection site with an evident cluster of grafted hNSCs (huN +) in the anterior horn of an animal at 40DAT. e At E-ST hNSCs (huN +) reached a higher grade of dispersion into the parenchyma. f, g Charts showing the differences in hNSCs migration (f) and survival (g) at 15, 40DAT or E-ST. ESS early-symptomatic stage, MSS mid-symptomatic stage, E-ST end stage, DAT days after transplantation, a.f. anterior funiculus, a.h. anterior horn. Scale bars: 100 μm

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