Fig. 3: PAX6 depresses sensitivity to cisplatin in human NSCLC cells by regulating stem cell transformation. | Cell Death & Disease

Fig. 3: PAX6 depresses sensitivity to cisplatin in human NSCLC cells by regulating stem cell transformation.

From: The PAX6-ZEB2 axis promotes metastasis and cisplatin resistance in non-small cell lung cancer through PI3K/AKT signaling

Fig. 3

a, b Expression of CD44, CD133, and ALCAM in the indicated groups of A549 and SPC-A-1 cells were detected by western blotting and immunofluorescence staining (scale bar, 50μm). c Representative images and quantitative analysis of sphere formation in the indicated groups of A549 cells (scale bar, 500μm). d mRNA levels of the stemness-associated genes, NANOG, SOX2, and OCT4, by RT-qPCR in the indicated groups of A549 cells. e Quantitative analysis of colony formation assays in the four groups of cells treated with four concentrations of cisplatin (0, 0.25, 0.5, 1μm). f CCK-8 assays were performed to determine the resistance or sensitivity to cisplatin at four concentrations; e, f: *P < 0.05, **P < 0.01; two-way ANOVA. g Quantitative analysis of flow cytometry results to detect apoptosis in the four groups of cells. **P < 0.01, ***P < 0.001; two-tailed Student’s t-test

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