Fig. 4: A subcutaneous tumor model for detecting cisplatin sensitivity in nude mice using A549 cells.

a Tumor volumes of subcutaneous Ctl or si-PAX6 or Vector or PAX6 overexpression xenografts during 21 days of treatment with PBS or CDDP. b, c Representative tumors isolated from nude mice and average tumor weights in the indicated groups; **P < 0.01, ***P < 0.001; two-tailed Student’s t-test. d IHC staining of PAX6, EMT-associated genes (E-cadherin, N-cadherin, vimentin, FSP-1), stem cell biomarkers (CD44, CD133, ALCAM), and the proliferation marker Ki67 in subcutaneous tumors of mice injected with Ctl vs. si-PAX6, Vector vs. PAX6 (scale bar, 50μm)