Fig. 5: Knockdown of PRMT1 attenuates tumour initiating cell-like properties of TICs. | Cell Death & Disease

Fig. 5: Knockdown of PRMT1 attenuates tumour initiating cell-like properties of TICs.

From: PRMT1 regulates the tumour-initiating properties of esophageal squamous cell carcinoma through histone H4 arginine methylation coupled with transcriptional activation

Fig. 5

a Western blot analysis showed the stable knockdown of PRMT1 by infection with lentivirus LV-shPRMT1 1# and 2# compared with the LV-GFP group. b–f qRT-PCR, flow cytometric, tumour spheroid formation assay, respectively, showed that knockdown of PRMT1 lead to down-regulation of stem cell-associated genes, the percentage of OV6+ subpopulation and capability of spheroid formation. Data are shown as the mean ± SD, *P < 0.05, **P < 0.01. All experiments were performed in triplicates. g, j NOD/SCID mice were injected with the indicated number of LV-shPRMT1-or LV-GFP-ECA109 OV6+ cells and tumour incidence in mouse xenografts was obtained after 28 days. h, j Representative tumours from mice injected with 5 × 104 LV-shPRMT1 or LV-GFP ECA109 OV6+ cells after 6 weeks are presented. Tumour volumes from each group were also measured weekly. i Immunohistochemistry analysis showed the PRMT1 and OV6 expression on mouse subcutaneous tumours inoculated after 6 weeks. Representative image is shown (scare bar = 100 µm). k Western blot analysis revealed that exogenous PRMT1 expression level was decreased in subcutaneous mouse tumours

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