Fig. 4: MALAT1 functions as a miR-34a sponge in A375 melanoma cells.

a Luciferase reporter constructs: Wild-type MALAT1 (wt-MALAT1) and MALAT1 with mutations in the miR-34a-binding sites (Luc-MALAT1-mut) were inserted into the psiCHECK-2 vector. Letters in bold font represent mutation sites. b Relative luciferase activity of A375 cells with psiCHECK-2 containing wt-MALAT1 co-transfected with control miRNA (miR-NC), miR-34a, or mutated miR-34a. c Relative luciferase activity of A375 cells with psiCHECK-2 containing wt-MALAT1 co-transfected with control miRNA (anti-miR-NC), anti-miR-34a, or mutated miR-34a (anti-miR-34a-mut). d Relative luciferase activity of A375 cells with psiCHECK-2 containing wt-MALAT1 and mutated MALAT1 (Luc-MALAT1-mut) co-transfected with miR-34a. e Relative luciferase activity of A375 cells with psiCHECK-2 containing wt-MALAT1 and mutated MALAT1 (Luc-MALAT1-mut) co-transfected with anti-miR-34a. f Relative luciferase activity of A375 cells with psiCHECK-2 containing c-Myc co-transfected with miR-34a and wt-MALAT1 or mutated MALAT1 (MALAT1-mut) expression vectors. g Relative luciferase activity of A375 cells with psiCHECK-2 containing Met co-transfected with miR-34a and wt-MALAT1 or mutated MALAT1 (MALAT1-mut) expression vectors. h An Ago2 immunoprecipitation experiment was performed for A375 cells transfected with the control vector or MALAT1 expression vector, followed by a quantitative real-time polymerase chain reaction assay to analyze the MALAT1, GAPDH, c-Myc, and Met associated with Ago2