Fig. 2: Analysis of engraftment and differentiation of BMCs in Rag2−/− mice.
From: Fibrotic liver microenvironment promotes Dll4 and SDF-1-dependent T-cell lineage development
a Flow cytometric analysis for the expression of CD25 and CD44 in thymocytes on day 28 after CD45.1 BMT through the hepatic portal vein. Differentiation of CD45.1 donor cells can be detected (left). b Flow cytometric analysis for the expression of CD25 and CD44 on thymocytes for DN1–DN4 stages of T-cell development in thymus of Rag2–/– mice with and without CCl4 treatment on day 28 after CD45.1 BMT. c Flow cytometric analysis for the expression of CD4 and CD8 in thymocytes for DP and SP stages of T-cell development in the thymus of Rag2−/− mice with or without CCl4 treatment on day 28 after CD45.1 BMT. d Flow cytometric analysis for the expression of CD4 and CD8 in lymphocytes for CD4+ and CD8+T cells in peripheral blood and spleen of Rag2−/− mice with or without CCl4 treatment on day 28 after CD45.1 BMT. The results are presented as mean ± S.E.M. Statistical significance was determined by Student’s t test. Significance between samples is indicated in the figures as follows: *P < 0.05; **P < 0.01
