Fig. 2: Pterostilbene (PT) induces autophagy in hepatocellular carcinoma (HCC) cells. | Cell Death & Disease

Fig. 2: Pterostilbene (PT) induces autophagy in hepatocellular carcinoma (HCC) cells.

From: Inhibition of eIF2α dephosphorylation accelerates pterostilbene-induced cell death in human hepatocellular carcinoma cells in an ER stress and autophagy-dependent manner

Fig. 2

a Huh-7 and SK-Hep-1 were treated with PT (0, 25, 50 and 100 μM) for 24 h, followed by acridine orange (AO) staining and visualisation under a fluorescence microscope. b Transmission electron microscope (TEM) image of the ultrastructure of SK-Hep-1 and Huh-7 cells treated with or without PT (100 μM) for 24 h. Scale bar: 5 μm. c Quantitative analysis of AO-stained cells using a flow cytometer. d Protein expression levels of Beclin-1, p62, LC3-II and pro-caspase-3 were assessed using Western blot analysis. β-Actin was employed as an internal control. **p < 0.01 compared with controls. *p < 0.05

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