Fig. 2: CLQ inhibits the proliferation of melanoma cells and suppresses tumor growth in vivo. | Cell Death & Disease

Fig. 2: CLQ inhibits the proliferation of melanoma cells and suppresses tumor growth in vivo.

From: Cloxiquine, a traditional antituberculosis agent, suppresses the growth and metastasis of melanoma cells through activation of PPARγ

Fig. 2

B16F10 and A375 cells were treated with the indicated concentrations of CLQ for 24 h. a EdU incorporation assay used to measure cell proliferation. b Protein expression levels of key regulators involved in cell cycle progression. c Quantification for the immunoblots from b. All values are presented as the mean ± SD from at least three separate experiments. *P < 0.05 and **P < 0.01 vs. CTL. B16F10 cells (1 × 106 cells per mouse) were injected subcutaneously into the right flank of nude mice. When tumors became palpable on day 7, mice were randomly divided into three groups and were administered either vehicle (olive, i.p., daily) or CLQ (5 mg/kg, 25 mg/kg, i.p., daily) for 8 days. n = 5 for each group. d Subcutaneous tumor volumes were measured at the indicated days. e Representative tumor images. f Tumor weights. g H&E staining and immunohistochemistry analysis of Ki-67 from tumor sections. All values are presented as the mean ± SD. *P < 0.05 and **P < 0.01 vs. CTL

Back to article page