Fig. 2: Effect of SASH1 on cell adhesion.
From: HMGB1 contributes to SASH1 methylation to attenuate astrocyte adhesion
A The results of GO analysis of the differential gene expression due to depletion of SASH1 were clustered in the biological adhesion and other biological processes categories. B The results of the cell adhesion assay showed that depletion of SASH1 expression attenuated cell adhesion when astrocytes were seeded on Laminin (Ln)-coated wells for 20 min. siSASH1 vs. siControl, n = 3, P = 0.017. C Overexpression of SASH1 in C6 cells significantly increased cell adhesion when C6 cells were seeded on Ln-coated wells for 20 min. Adv4-SASH1 vs. Adv4-NC, n = 3, P = 0.011. D The results of the cell invasion assay showed that depletion of SASH1 expression markedly increased cell invasion when astrocytes were seeded on Ln-coated upper chambers. a Representative images of the transwell assay results. b The statistical results. siSASH1 vs. siControl, n = 4, P = 0.0283. E Overexpression of SASH1 in C6 cells significantly decreased the ability of C6 cells to invade the Ln-coated upper chambers. a Representative images of the transwell assay results. b The statistical results. Adv4-SASH1 vs. Adv4-NC, n = 4, P = 0.0324
