Fig. 4: High methylation levels of the SASH1 gene in C6 glioma cells.
From: HMGB1 contributes to SASH1 methylation to attenuate astrocyte adhesion
A Schematic of a CpG island showing the locations of the 17 CpG sites (shown in red text) in the 5′ UTR of the SASH1 gene promoter. B Representative PCR results for the SASH1 promoter region using primers specific for methylated (M) and unmethylated (U) gDNA in astrocytes (AS) and C6 cells. The bar graph (lower panel) shows the M-to-U product ratios. C6 vs. AS, n = 3, P = 0.0194. C Each square indicates a clone of AS or C6 cells. Six clones were subjected to bisulfite sequencing. The clones methylated on individual CpG sites are labeled in black. D A significant increase in the percentage of methylated CpG sites was observed in C6 cells compared to AS cells. P = 0.0002
