Fig. 2: ASK induces cell cycle arrest through downregulation of key cell cycle progression factors. | Cell Death & Disease

Fig. 2: ASK induces cell cycle arrest through downregulation of key cell cycle progression factors.

From: Mitochondrial ncRNA targeting induces cell cycle arrest and tumor growth inhibition of MDA-MB-231 breast cancer cells through reduction of key cell cycle progression factors

Fig. 2

a MDA-MB-231 cells were transfected with 200 nM Andes-1537, ASO-C or left untreated (NT) for 24 h, stained with PI and cell cycle distribution was analyzed by flow cytometry. b A triplicate analysis of the experiment in a shows accumulation of cells in S phase and a decrease in the G2/M population, compared to controls (ASO-C vs. Andes-1537; two-tailed Student’s t-test, ***p < 0.001). c Representative results of Western blot analysis of cells treated as in a for 24 h. Surv, survivin; β-act, β-actin. Quantification of three independent experiments (n = 3) is shown for cyclin B1 (d), cyclin D1 (e), cyclin A1 (f), cyclin E1 (g), CDK1 (h), CDK4 (i) and survivin (j). d–j two-tailed Student’s t-test; (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ASO-C vs. Andes-1537). All graphs represent Mean ± S.E.M.

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