Fig. 3: The miRNAs hsa-miR-4485-5p, hsa-miR-4485-3p and hsa-miR-1973 are upregulated by ASK.

a Scheme depicting sequence and position of hsa-miR-4485-5p (pink), hsa-miR-4485-3p (teal), and hsa-miR-1973 (green) on the IR of ASncmtRNA-2. The A in brown represents a base that is contained in hsa-miR-4485-3p as well as in hsa-miR-1973. b Relative quantification of miRNAs by RT-qPCR (Taqman assay) is shown for hsa-miR-4485-5p, hsa-miR-4485-3p and hsa-miR-1973, in a triplicate analysis of three independent experiments (n = 3), in untreated cells (NT) or cells transfected with Andes-1537 or ASO-C at 24 h post-transfection (two-tailed Student’s t-test; Mean ± S.E.M. **p < 0.01; ****p < 0.0001; ASO-C vs. Andes-1537)