Fig. 4: Transfection of MDA-MB-231 cells with hsa-miR-4485-3p mimic induces downregulation of cyclin B1 and cyclin D1. | Cell Death & Disease

Fig. 4: Transfection of MDA-MB-231 cells with hsa-miR-4485-3p mimic induces downregulation of cyclin B1 and cyclin D1.

From: Mitochondrial ncRNA targeting induces cell cycle arrest and tumor growth inhibition of MDA-MB-231 breast cancer cells through reduction of key cell cycle progression factors

Fig. 4

MDA-MB-231 cells were transfected for 48 h with control mimic (C), hsa-miR-1973 mimic (1973) or hsa-miR-4485-3p mimic (4485-3p) (n = 3). a miRNA upregulation was confirmed by Taqman RT-qPCR assays (two-tailed Student’s t-test; ****p < 0.0001; C vs. each specific mimic) (n = 3). b Relative total number of cells (two-tailed Student’s t-test; **p < 0.01, ***p < 0.001; C vs. each specific mimic; n = 3). c Representative results of Western blot analysis in mimic-transfected cells. Surv, survivin; β-act, β-actin. d–h, Graphical representation of the results of three independent experiments (n = 3) for cyclin B1 (d), cyclin D1 (e), survivin (f), CDK1 (g) and CDK4 (h) (two-tailed Student’s t-test; *p < 0.01, ***p < 0.001; C vs. each specific mimic). All graphs represent Mean ± S.E.M.

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