Fig. 5: ASK induces increase of nuclear-encoded miRNAs, which regulate CDK1 expression. | Cell Death & Disease

Fig. 5: ASK induces increase of nuclear-encoded miRNAs, which regulate CDK1 expression.

From: Mitochondrial ncRNA targeting induces cell cycle arrest and tumor growth inhibition of MDA-MB-231 breast cancer cells through reduction of key cell cycle progression factors

Fig. 5

a Forty-eight hour after transfection of ASO-C or Andes-1537 or no treatment (NT), relative levels of hsa-miR-5096 and hsa-miR-3609 were determined by RT-qPCR (Taqman assays) (two-tailed Student’s t-test; **p < 0.01; ASO-C vs. Andes-1537; n = 3). b Putative binding sites for hsa-miR-5096 and hsa-miR-3609 on the mRNA 3’UTR of CDK1 and hsa-miR-5096 on CDK4, determined by TargetScanHuman. Bold lines represent potential 5’ seed region binding; solid lines depict Watson–Crick base pairing, broken lines show G:U wobble. c Relative quantification of hsa-miR-5096 and hsa-miR-3609 after transfection of mimics (two-tailed Student’s t-test; **p < 0.01; C vs. each specific mimic; n = 3). d Representative results of Western blot analysis for CDK1 and CDK4, using β-actin as loading control, in mimic-transfected cells at 48 h. e, f Graphical representation of triplicate analyses of the results shown in d, for CDK1 (e) and CDK4 (f) (two-tailed Student’s t-test; *p < 0.01; C vs. each specific mimic; n = 3). All graphs represent Mean ± S.E.M.

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