Fig. 3: ASH2L is involved in ZNF479-suppressed MT-1 expression. | Cell Death & Disease

Fig. 3: ASH2L is involved in ZNF479-suppressed MT-1 expression.

From: ZNF479 downregulates metallothionein-1 expression by regulating ASH2L and DNMT1 in hepatocellular carcinoma

Fig. 3

Huh-7 cells were transfected with control/ZNF479 overexpression vectors or scramble/ZNF479 siRNAs for 48 h. a Expression of H3K4me2, H3K4me3, Acetyl-H3, Histone H3, Acetyl-H4, and Histone H4 was examined by western blot analysis in control/14-3-3ε–overexpressing cells (left), vector/ZNF479-transfected cells (middle) and scramble/ZNF479 siRNA-transfected cells (right). b–e Expression of ASH2L and Menin was examined by western blot and qPCR analysis in control/14-3-3ε–overexpressing cells (b, d, left), vector/ZNF479-transfected cells (right) and scramble/ZNF479 siRNA-transfected cells (c, e). f, g Huh-7 cells were transfected with ASH2L and Menin siRNAs for 48 h. Relative expression of ASH2L, Menin and MT-1 was determined by western blot analysis. Gene expression of qPCR analysis was normalized to both control cells as well as GAPDH. Actin was used as a loading control for western blotting analysis. Scale bars: mean ± SD. **P < 0.01

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