Fig. 3: TC2N impedes PI3K-AKT signaling by blocking ALK-induced p55γ phosphorylation in BC cells.
From: Identification of TC2N as a novel promising suppressor of PI3K-AKT signaling in breast cancer
a TCGA BC RNA-seq dataset identified the top 10 categories of the GO signaling pathway that associate with TC2N expression. b, c WB analysis of PI3K-AKT signaling-related protein level in stable transfected BC cells. d The stable transfected BC cells were transfected with negative control or ALK siRNA for 48 h and then the cells were lysed and were subjected to WB using indicated antibodies. ACTIN serves as an internal control. e, f The stable transfected BC cell lysates were subjected to IP using p55γ or ALK antibodies and then detected by WB using ALK or p55γ antibodies. Normal IgG serves as a negative control. Whole-cell lysates were used as a positive control (Input)
