Fig. 1: Aurora A regulates the protein expression and transcription activity of YAP.
From: Activation of Aurora A kinase increases YAP stability via blockage of autophagy
a Western blot analysis of the protein levels of Aurora A and YAP in eight pairs of clinical lung cancer (T) and adjacent normal tissues (N). Statistical analysis of Aurora A and YAP expression levels normalized to relative glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (R2 = 0.8720, ***P < 0.001). b Human lung cancer tissues were analyzed by immunohistochemical staining with Aurora A and YAP antibody. Representative images were presented. Scale bars, 100 μm. c The correlation between Aurora A and YAP expression in lung cancer tissues from 43 patients. ***P < 0.001. d Western blot analysis of YAP protein expression in A549 cells with Aurora A knockdown (shA1 or shA2). e Western blot analysis of YAP protein expression in A549 cells with Aurora A overexpression (AURKA). f Aurora A depleted A549 cells were transfected with plvx or plvx-AURKA plasmids. Western blot analysis of YAP protein expression. g Luciferase reporter assay to evaluate the activity of YAP from A549 cells with Aurora A knockdown(shA1). Error bars represented mean ± S.D. (n = 3, **P < 0.01). h RT-qPCR analysis of the mRNA levels of YAP target genes (CTGF, CYR61) in shAURKA (shA1) and shNC A549 cells. Error bars represented mean ± S.D. (n = 3, **P < 0.01). i Western blot analysis of the protein levels of YAP and its target gene CTGF in shAURKA (shA1) and shNC A549 cells
