Fig. 2: Aurora A regulates the protein expression and transcription activity of YAP through its kinase activity.
From: Activation of Aurora A kinase increases YAP stability via blockage of autophagy
a Western blot analysis of YAP protein level in A549 cells treated with indicated doses of VX-680 for 24 h. b Western blot analysis of YAP protein level in Aurora A overexpressed (AURKA) A549 cells incubated with VX-680 (200 nM) or DMSO for 24 h. c Western blot analysis of YAP protein level in A549 cells transfected with wild-type Aurora A(A-WT) and plasmids encoding different kinase forms of Aurora A (A-T288D, A-D274N) for 48 h. d Luciferase reporter assay to evaluate the activity of YAP from A549 cells treated with VX-680(200 nM) or DMSO for 24 h. Error bars represented mean ± S.D. (n = 3, **P < 0.01). e RT-qPCR analysis of the mRNA levels of YAP target genes in A549 cells treated with VX-680 (200 nM) or DMSO for 24 h. Error bars represented mean ± S.D. (n = 3, **P < 0.01). f Western blot analysis of the protein levels of YAP and its target gene CTGF in A549 cells treated with different doses of VX-680 for 24 h. g RT-qPCR analysis of YAP mRNA level in shAURKA (shA1) cells and control groups. Error bars represented mean ± S.D. (n = 3). h RT-qPCR analysis of YAP mRNA levels in A549 cells treated with VX-680 (200 nM) or DMSO. Error bars represented mean ± S.D. (n = 3)
